Visit the I NTEGRA Biosciences website to learn more, or use the Request Information button to make direct contact. or the extraction sample preparation method (SOP 1867813 Cultivation and Sample. equimolar library was constructed by pooling samples, and the resulting library was sent for sequencing on an Illumina MiSeq platform at Microsynth AG. In my opinion, INTEGRA handheld electronic pipettes are impressive as they solved the complications in our lab, and I truly believe they outperform 1, 8, and 12 channel pipettes – I love it!” Sanger sequencing was performed by Microsynth Seqlab GmbH (Goettingen. Further processing of the samples was conducted by Microsynth (Balgach, Switzerland). Being able to keep a close eye on the speed of liquid aspiration and dispensing helps to avoid any loss, and reduces the likelihood of poor sample recovery, ensuring that quality standards are matched and expectations are met. Genomic DNA extraction was performed as described above. The VIAFLO 96, on the other hand, is used in cfDNA isolation to pipette samples with different viscosities over a wide volume range, which would otherwise be difficult to program on automated systems. All these applications require precise, simultaneous transfer of multiple liquid samples the MINI 96 has allowed us to do exactly this and, best of all, we get more hands-off time. Microsynth is a leading European company in the area of nucleic acid synthesis and analysis. The typical workflow for amplicon-sequencing data analysis comprises demultiplexing of the dataset in order to assign sequences to samples and merging of paired-end reads to obtain single sequences (however, there are also situations where single-end reads are provided or where merging is not recommended for some reason). “We use the MINI 96 for digital PCR and qPCR preparation, as well as in end-point PCR reactions and library preparation for NGS. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins. We use the MINI 96 and VIAFLO 96 in every step of sample preparation to achieve this high throughput.” Applications Nanopore sequencing offers advantages in all areas of research. Scientists at Microsynth – a leading European company in nucleic acid synthesis, analysis, and sequencing – are taking advantage of the remarkable liquid handling capabilities of INTEGRA’s MINI 96 and VIAFLO 96 handheld electronic pipettes in workflows ranging from nucleic acid isolation to sequencing and different types of PCR.ĭr Sergey Yakushev, Head of Genetic Analysis at Microsynth, explained: “We receive as many as 20,000 samples per year from our customers, so we do a lot of preparation and analysis that requires us to perform multiple liquid handling steps.
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